FIGURE

Fig. 4

ID

ZDB-FIG-191018-12

Publication

Oikonomou et al., 2019 - The Serotonergic Raphe Promote Sleep in Zebrafish and Mice

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Fig. 4

Optogenetic Stimulation of the Raphe Results in Reduced Locomotor Activity and Increased Sleep in Zebrafish

(A–C′) Maximum intensity projections of brains (A–C) and single plane images of pineal glands (A′–C′) from 5 dpf Tg(tph2:ChR2-YFP) zebrafish immunostained for 5-HT and YFP. Scale bars, 50 μm. a, anterior; p, posterior; Pt, pretectal area; Hyp, hypothalamus; Ra, raphe.

(D and E) Left: normalized locomotor activity (D) and sleep (E) of 5 dpf Tg(tph2:ChR2) (red) and non-transgenic sibling control (blue) zebrafish before and during exposure to blue light. Right: normalized locomotor activity (D) and sleep (E) fold change during illumination.

(F and G) Same as (D) and (E) but in tph2^−/− animals.

n, number of animals; 6 trials per animal. ns p > 0.05, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001, Mann-Whitney test.

Expression Data

Gene:	YFP
Fish:	ct865Tg
Anatomical Term:	raphe nucleus
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ct865Tg tph2^ct817/ct817; ct865Tg
Condition:	visible light
Observed In:	locomotory behavior sleep
Stage:	Day 5

Phenotype Detail

Acknowledgments

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