Fig. 7

Ginsenoside F1 increased postischemic angiogenesis and improved focal cerebral blood perfusion in MCAO rats. (A–B) GF1 promoted postischemic angiogenesis and increased the MVD in the ipsilateral hemisphere of MCAO rats. At the 7th and 14th day after MCAO, the rats were anesthetized, transcardially perfused with 4% paraformaldehyde, and the whole brains were removed. Then, the 10 μm brain cryosections were stained with anti-CD31 and nucleus was stained with DAPI. Images of immunofluorescence staining were obtained and the quantification of the MVD is shown. GF1 markedly increased the relative D^* (C–D) and f values (E–F) in the ipsilateral hemisphere at the 7th and 14th day after MCAO. Rats were received intragastrically administeratioin of GF1 (50 mg/kg) at 24 h after MCAO once daily for 14 days. All rats were anesthetized, and MRI scans were recorded at the 1st, 7th and 14th day after MCAO. The D^* and f values were quantitatively calculated from the IVIM-DWI at the postprocessing workstation. Quantification of relative D^* (D) and f values (F) and the corresponding parameter maps are shown. Representative images are shown; scale bar: 50 μm (A); NS represents no significance. The data are presented as mean ± SEM (n = 6). ^*P <  0.05 and ^**P <  0.01 compared with the MCAO group.