Fig. 5

Co-registration of FuGIMA with Two Functional Imaging Datasets Shows Overlap of DS-RGC Input with Monocular DS, but Not with Translation-Selective, Neurons

(A) Schematic illustrating acquisition and integration of the functional maps and the FuGIMA dataset.

(B–I) A slice of the co-registered volume at the level of the AFs 4–6 with FuGIMA tracings and functional maps of DS-RGC terminals and DS neurons (right hemisphere, maximum intensity projection over z = 10 μm; see schematic in F; of 58 FuGIMA tracings, 42 of the following classes extend into the slice: 19 monocular DS; 17 translation-selective; 6 not motion-sensitive).

(B–E) FuGIMA tracings (open white arrowhead, FuGIMA tracing bundle; filled white arrowhead, small tracing patch at the border between AFs 5 and 6; open arrow, direction of oblique view): (B) all (white); (C) monocular DS (green); (D) translation-selective (magenta); and (E) non-motion-sensitive (blue).

(F) Schematic of z stack slicing (oblique view used to visualize optic tract [light gray] and AFs [dark gray]).

(G) Registered 3D map of DS-RGC terminals (isl2b:Gal4, UAS:syGCaMP6s; see color wheel below for direction of moving gratings presented from side; composite of 6 fish).

(H) Registered 3D map of DS-panneuronal (HuC:GCaMP5G; white arrow, broad band of DS pixels; see color wheel below for direction of moving gratings presented from below; composite of 5 fish).

(I) Composite of DS-panneuronal with all FuGIMA tracings and standard brain reference marker (HuC:lyn-tagRFP in gray).

For (G)–(I), imaging artifact DS pixels located in the eye were removed with a mask. Scale bar represents 50 μm (I).