Fig. S1

Variations in sl19 phenotype in Tg(p2rx3.2:gfp) embryos. sV (also labeled as llf), sVII, sIX and sX are the projections of the trigeminal (gV), facial (gVII), glossopharyngeal (gIX) and vagus (gX) ganglia, respectively. Panel (A) shows wild type p2rx3.2:gfp. Panel (B) is a schematic showing the ganglia and their projections in matching colors. Panels (C–F) are images from individual sl19 mutants. Panel (C) shows sVII defasciculating, with the majority of fibers stopping before reaching the plexus and the few fibers that do reach the plexus do so in an aberrant fashion. sIX joins sX before it enters the plexus, and sX shows aberrant branching. In (D), the main bundle of sIX stops in the hindbrain while a small number of fibers make a caudal turn towards the plexus. sX also shows a small degree of defasciculation by individual axons. Panel (E), sVII defasiculates and sIX continues in an anterior direction towards sVII. sX fails to branch properly but does contribute to the plexus. Panel (F), sVII again defasciculates and fails to reach the region of the plexus, sIX stops in the hindbrain and fails to form a terminal field, and sX shows abnormal branching and also fails to form a normal terminal field. Panel (G) is a schematic showing a selection of the aberrant phenotypes seen in sl19 mutants. All images are confocal z-stacks oriented anterior to the left and dorsal at top. Scale bars = 100 μm.