PUBLICATION

The Metalloproteinase adam19b Is Required for Sensory Axon Guidance in the Hindbrain

Authors
Cox, J.A., Voigt, M.M.
ID
ZDB-PUB-190322-6
Date
2019
Source
Frontiers in neural circuits   13: 14 (Journal)
Registered Authors
Cox, Jane, Voigt, Mark
Keywords
axon guidance, cranial nerves, metalloprotease, sensory circuits, zebrafish
MeSH Terms
  • Ganglia, Invertebrate/cytology
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Receptors, Purinergic P2/genetics
  • Receptors, Purinergic P2/metabolism
  • ADAM Proteins/genetics
  • ADAM Proteins/metabolism*
  • Axon Guidance/genetics
  • Axon Guidance/physiology*
  • Larva
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Gene Expression Regulation, Developmental/physiology
  • Animals, Genetically Modified
  • Zebrafish
  • Animals
  • Embryo, Nonmammalian
  • Sensory Receptor Cells/metabolism*
  • DNA Mutational Analysis
  • Mutation/genetics
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Rhombencephalon/cytology*
  • Rhombencephalon/physiology*
  • CRISPR-Associated Protein 9/genetics
  • CRISPR-Associated Protein 9/metabolism
(all 25)
PubMed
30894803 Full text @ Front. Neural Circuits
Abstract
Little is known about the molecular and cellular mechanisms involved in the formation of the cranial peripheral sensory system in vertebrates. To identify genes involved in the formation of these circuits, we performed a forward genetic screen utilizing a transgenic zebrafish line (p2rx3.2:gfpsl1) that expresses green fluorescent protein (gfp) in sensory neurons of the Vth, VIIth, IXth and Xth cranial ganglia. Here, we describe a novel zebrafish mutant in which a missense mutation in the adam19b gene selectively affects the epibranchial sensory circuits.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sl1TgTransgenic Insertion
    sl8TgTransgenic Insertion
      sl9TgTransgenic Insertion
        sl17TgTransgenic Insertion
          sl19
            Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            adam19bCRISPR1-adam19bCRISPR
            adam19bMO1-adam19bMRPHLNO
            adam19bMO2-adam19bMRPHLNO
            1 - 3 of 3
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            Fish
            Antibodies
            No data available
            Orthology
            Gene Orthology
            adam19b
            1 - 1 of 1
            Show
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            mCherryEFGmCherry
            NTREFGNTR
            1 - 3 of 3
            Show
            Mapping
            No data available