FIGURE

Fig. 1

ID

ZDB-FIG-190910-22

Publication

O'Connor et al., 2019 - Modulation of Agrin and RhoA Pathways Ameliorates Movement Defects and Synapse Morphology in MYO9A-Depleted Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Phenotypes of myo9aa/ab crispant zebrafish. (A) Representative images of 48 hpf zebrafish injected with sgRNAs against myo9aa and myo9ab (numbers following the gene refer to exon targeted). Injection of a combination of all four sgRNAs was performed (myo9aa/ab), along with a control against the tyrosinase gene (tyr) in which no pigment can be observed, confirming knockdown success. Scale bar = 500 µm. (B) Images showing phenotypic classification of injected zebrafish at 72 hours post fertilisation (hpf), ranging from normal to severe. Graph shows proportion of myo9aa/ab crispant fish injected with all four sgRNAs (n = 7, including 160 fish in total) as compared to wild-types (black line, n = 7, including 138 fish in total). Error bars represent mean ± S.E.M, ** p < 0.01, paired t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-myo9aa WT + CRISPR1-myo9ab WT + CRISPR2-myo9aa WT + CRISPR2-myo9ab WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab
Knockdown Reagents:	CRISPR1-myo9aa CRISPR1-myo9ab CRISPR2-myo9aa CRISPR2-myo9ab
Observed In:	heart whole organism
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells