Fig. 4

Brainbow expression does not cause clonal apoptosis.

A) The brightness of cells that undergo apoptosis paired with the brightest neighboring control cell; brightness is quantified by summed relative RGB value (range of 0 to 3; n=30?cells/condition from 4 fish). Solid black lines represent brightness differences when the dying cell is part of a clonal apoptosis event and dashed red lines represent cases in which the dying cell is an isolated death. Arrowheads show the overall range of healthy control cells in the same tissue. B) Mean number of apoptotic events observed in hindbrain over time imaged; error bars represent standard error of the mean; n=4 fish. C) Quantified activated caspase-positive cells in fish expressing Brainbow following injection and heat shock, and in uninjected, wild-type fish at both 1 dpf (Brainbow: n=23 fish, WT: n=16 fish) and 2 dpf (Brainbow: n=29 fish, WT: n=40 fish). Whiskers represent extremes of data. D) 48 hpf zebrafish hindbrain expressing Brainbow (maximum intensity projection of 29μm); caspase staining is coded as white for display. Dorsal is up and rostral is to the left; scale bar represents 20μm. In D, dTomato is coded as red, YFP is coded as green, and CFP is coded as blue.