FIGURE

Fig. 4

ID

ZDB-FIG-190910-14

Publication

Witzigmann et al., 2019 - Optimization-by-design of hepatotropic lipid nanoparticles targeting the sodium-taurocholate cotransporting polypeptide

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Fig. 4

Targeting ability of Myr-preS2-31 conjugated nanoparticles in vivo in xenotransplanted zebrafish embryos.

Nanoparticles were modified with different amounts of Myr-preS2-31 and injected into wild type zebrafish embryos xenotransplanted with human, GFP expressing HEK293 cells (green signal), (A) deficient or (B) expressing SLC10A1. Membrane of nanoparticles was fluorescently labeled using DiI (red signal). Yellow signals demonstrate colocalization (i.e. binding and internalization) of nanoparticles with HEK293-GFP cells. Representative brightfield and fluorescence images of tail region 1 h post injection are shown. Quantitative analysis of nanoparticle binding to HEK293-GFP cells is represented by Pearson´s Correlation Coefficient (PCC). All values are shown as box plots of biological replicates (n ≥ 2 independent experiments). *p<0.05. Numerical data for all graphs are shown in Figure 4—source data 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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