FIGURE

Fig. 2

ID

ZDB-FIG-190822-45

Publication

Lombó et al., 2019 - Embryonic Exposure to Bisphenol A Impairs Primordial Germ Cell Migration without Jeopardizing Male Breeding Capacity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Epigenetic profile of PGCs and their somatic surrounding cells. (A) Quantification of 5 mC by UPLC-MS in DNA of genital ridges (GR) from control and BPA-treated embryos. Bars represent the percentage of 5mC relative to C in 3 pools of 20 genital ridges per treatment (n = 3). Quantification of H3K9ac by whole mount immunofluorescence in (B) germ cells and (C) somatic cells of 24 hpf control and BPA-exposed embryos. Boxes represent the median plus maximum and minimum of H3K9ac intensity relative to nuclear area in 4 independent experiments (n = 4). (D) confocal images of double immunostaining in genital ridges of 24 hpf control embryos. H3K9ac was labelled with AlexaFluor^®568 (red fluorescence), Vasa protein within PGCs was labelled with AlexaFluor^®488 (green fluorescence) and nuclei were stained with DAPI (in blue); scale bar 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules