FIGURE

Fig. 4

ID

ZDB-FIG-190820-65

Publication

Adeyemo et al., 2019 - ZRANB3 is an African-specific type 2 diabetes locus associated with beta-cell mass and insulin response

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Fig. 4

Knockdown of zranb3 reduces β-cell number in zebrafish larvae. Injection of antisense oligonucleotide morpholinos (MO) targeting zranb3 reduced β-cells in 5 dpf larval zebrafish, detected by Tg(ins:mCherry) expression imaged via whole-mount confocal microscopy (a, b). c Depth coding of confocal microscopy images of whole-mount islets reveals individual cell resolution of β-cells in 5 dpf larval islets, color coded by depth (distal to proximal) along the z-axis. d, eWhole-mount immunostaining of MO-injected Tg(ins:mCherry) larval islets using antibody against glucagon (green). f Quantification of β-cell number in 5 dpf larval islets of Tg(ins:mCherry) injected with indicated concentrations of MO against zranb. (n = 17–19). Bars represent average across groups. Error bars represent SEM. p Values are from the t test. Scale bars = 25 µM

Expression Data

Antibody:	Ab1-gcg
Fish:	jh2Tg + MO3-zranb3
Knockdown Reagent:	MO3-zranb3
Anatomical Term:	endocrine pancreas
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh2Tg + MO3-zranb3
Knockdown Reagent:	MO3-zranb3
Observed In:	pancreatic B cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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