FIGURE
Fig. 3
- ID
- ZDB-FIG-190820-64
- Publication
- Adeyemo et al., 2019 - ZRANB3 is an African-specific type 2 diabetes locus associated with beta-cell mass and insulin response
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Fig. 3
Generation of zranb3 genomic zebrafish mutants. a, b Identified mutations found in homozygous F2 animals by CRISPR/Cas9 in Tg(ins:mCherry) or wild-type animals. In both lines genomic DNA deletions in exon 4 resulted in frame shift and premature stop codons (red box), confirmed by sequencing. c Confocal microscopy images of ins:mCherry transgene (red) in principal islets of wild type or mutant 5 dpf larvae. d Confocal images of whole-mount immunostained islets of wild type or mutant 5 dpf larvae, using antibodies against insulin (red) or glucagon (green). Scale bars = 25 µM |
Expression Data
| Antibody: | |
|---|---|
| Fish: | |
| Anatomical Term: | |
| Stage: | Day 5 |
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
|---|---|
| Observed In: | |
| Stage: | Day 5 |
Phenotype Detail
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Full text @ Nat. Commun.