Fig. 2

In vivo visualization of basal keratinocytes and actinotrichia. (A) Peridermal cells expressing GFP-CaaX under the keratin4 promoter. (B) Merged image with (A) and basal keratinocyte expressing mCherry-CaaX under the and1 promoter. (C) Transverse section image of (B). (D–F) Fluorescence images of the visualized actinotrichia by And1^480bp-GFP. (D) The median fin fold in the larva 6?days post-fertilization (dpf). (E) The tip of caudal fin in 3-week-old fish. (F) The magnified image of white box in (E). Fin skeleton was stained by alizarin red (magenta). Each actinotrichia extended from the tip of fin-rays. The white dot lines show the actinotrichia orientation. (G) Immunofluorescence against Collagen II. (H) GFP fluorescence of the visualized actinotrichia by And1^480bp-GFP. (I) Merged image with (G) and (H). (G′), (H′) and (I′) Optical cross section images in (G), (H) and (I), respectively. Note that both of immunofluorescence against Collagen II and GFP fluorescence of And1^480bp-GFP are detected in all actinotrichia (G–I′). Scale bar is 50?μm in (A–F) and 20?μm in (G–I). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)