Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-190807-13
Talbot et al., 2019 - Muscle precursor cell movements in zebrafish are dynamic and require six-family genes. Development (Cambridge, England)   146(10) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. S5

Fins are not required for MMP specification. (A-D) Brightfield images of four-dayold embryos. Brightfield images of wild-type, dnFgfrexpressing, and Δsix1a;4a;Δsix1b;4b mutant embryos. Fins are present (asterisk) in wild-type (A) and Δsix1a;4a;Δsix1b;4b mutant (B) embryos, but absent in dnFgfr-induced (C) and aldh1a2 mutant (D) embryos (aldh1a2 mutant fin phenotype previously shown by Grandel et al., 2002). (E-J) RNA in situ hybridization for (E, F) met and (G, H) lbx1a in 36 hpf embryos. (E-H) Although migratory streams are severely misshapen in aldh1a2 mutants, MMP markers lbx1a (E, F) and met (G, H) are expressed at levels comparable to wild-type embryos. In aldh1a2-/- mutant MMPs, met expression appears darker than in WT (E, F); this may be due to greater cell density in the mutant, which has impaired migration. Embryos in A-C and E-H were PTU-treated to inhibit pigment formation. MMP arrowheads are color coded as described in Figures 1 and S1. Scale bars are 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lbx1a WT standard conditions Prim-25 anterior migratory muscle precursor stream ISH
Prim-25 middle migratory muscle precursor stream ISH
Prim-25 posterior migratory muscle precursor stream ISH
aldh1a2i26/i26 standard conditions Prim-25 anterior migratory muscle precursor stream ISH
Prim-25 posterior migratory muscle precursor stream ISH
met WT standard conditions Prim-25 anterior migratory muscle precursor stream ISH
Prim-25 middle migratory muscle precursor stream ISH
Prim-25 posterior migratory muscle precursor stream ISH
aldh1a2i26/i26 standard conditions Prim-25 anterior migratory muscle precursor stream ISH
Prim-25 posterior migratory muscle precursor stream ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
Df(Chr20:six1b,six4b)oz16/oz16; Df(Chr13:six1a,six4a)oz27/+; cz3327Tg; oz5Tg standard conditions Day 4 pectoral fin present, normal
aldh1a2i26/i26; cz3327Tg; oz5Tg standard conditions Day 4 pectoral fin absent, abnormal
cz3327Tg; oz5Tg; pd1Tg/pd1Tg standard conditions Day 4 pectoral fin absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development