Fig. 3

Anti-inflammatory reagents and cisapride reduce leukocyte and apoptotic cells in the heart of bre mutant zebrafish. (A). Representative heart sections of DMSO, Dex (100 μm), MMP in (1 μm) and Cis (10 μm)-treated bremutant zebrafish at 1 dpc and 4 dpc showing the L-plastin positive cells (red, white arrowhead) in the injured heart area (bounded by white dashed line). Scale bar: 100 μm. (B). Bar chart shows the quantification of L-plastin positive cells (n = 3–5) in panel A was significantly different at p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***), one-way ANOVA. (C). Anti-inflammatory reagents and cisapride reduce apoptosis in bre mutant heart after injury. Representative heart sections of DMSO, Dex (100 μm), MMP in (1 μm) and Cis (10 μm) treated bremutant at 1 dpc and 4 dpc show the apoptotic cells (red, white arrowhead) in the injured area. Scale bar: 100 μm. (D). Bar chart shows the quantification of apoptotic cells (n = 3–5) in panel C was significantly different at p < 0.05 (*) and p < 0.001 (***), one-way ANOVA. (E, F). qRT-PCR analysis shows the expression of inflammatory genes (E) and apoptotic/anti-apoptotic genes (F) in bre mutant at 4 dpc with different anti-inflammatory reagents and cisapride regimens. Asterisks indicate a significant difference observed between DMSO control and Dex (100 μm), MMP in (1 μm) or Cis (10 μm) treatment at p < 0.05 (*) and p < 0.01 (**), one-way ANOVA.