FIGURE

Figure 3

ID

ZDB-FIG-190723-684

Publication

Kiselev et al., 2019 - Truncating Variant in Myof Gene Is Associated With Limb-Girdle Type Muscular Dystrophy and Cardiomyopathy

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Figure 3

Loss of Myof affects the skeletal muscle of zebrafish embryos. (A)myof knockdown was achieved by injecting a splice-target morpholino (myof-MO). (B) Loss of Myof results in wavy and abnormally patterned myofibers (Myosin, red), and disruption of the myosepta, seen by a decreased intensity in the staining and increased width (Dystrophin and Dystroglycan, green). (C)Analysis of the myofiber ultrastructure by electron microscopy shows disruption of myofiber structure (C4) and their sarcomeres (arrows, C5, C6), abnormally shaped and distributed mitochondria (arrowheads, C5), when compared to control mitochondria (arrowheads, C1), and Z-disc thickening (arrowheads, C6).

Expression Data

Genes:	dag1 dmd
Antibodies:	Ab1-dmd Ab4-dag1
Fish:	WT + MO1-myof
Knockdown Reagent:	MO1-myof
Anatomical Term:	myoseptum
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-myof
Knockdown Reagent:	MO1-myof
Observed In:	myoseptum skeletal muscle myoseptum skeletal muscle skeletal muscle myofibril skeletal muscle cell mitochondrion
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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