Fig. 3.

Interaction of the predatory bacteria Bdellovibrio with Shigella in the zebrafish. (A) Wild-type zebrafish larvae were injected in the hindbrain ventricle (HBV) at 3 days postfertilisation with >5×10³ colony forming units (CFUs) of GFP-S. flexneri (green), followed by hindbrain injection of either PBS or 1-2×10⁵ plaque forming units (PFUs) of mCherry-Bdellovibrio (red), 30-90 min after the initial Shigella infection. Representative images of the HBV in PBS- or Bdellovibrio-treated zebrafish larvae infected with Shigella are shown. The dashed line box shows the region of interaction between fluorescent Bdellovibrio and Shigella. For both treatments, the same larva was imaged over time. Scale bar: 100 µm. hpi, hours postinfection. (B) Representative images of Shigella predation by Bdellovibrio in vivo imaged by high-resolution confocal microscopy. Frames captured over time show stages of Bdellovibrio (red) invasive predation and rounding of Shigella (green). Scale bar: 2.5 µm. mpi, minutes postinfection. Adapted from Willis et al. (2016).