FIGURE

Fig 1

ID

ZDB-FIG-190723-1662

Publication

Zhou et al., 2018 - Distinct amino acid motifs carrying multiple positive charges regulate membrane targeting of dysferlin and MG53

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Fig 1

Accumulation of smDysf at the lesion site depends on the positive charge of the WRRFK motif <italic>in vivo</italic>.

(A) Domain structure of Dysf. The boxed fragment is smDysf, which was used to test accumulation at the lesion patch. (B) Representative images showing accumulation of smDysf and different mutants. Basic AAs are highlighted in red; arrows indicate the site of lesion. Z-line (z) and sarcolemmal (sc) regions are noted. (C-D) Corresponding kinetics of accumulation of zebrafish (C) or human (D) smDysf at the damage site, again with the basic AAs highlighted in red. The fluorescence intensity at the lesion was normalized to the one of the undamaged state. Intensity courses are averages over 9–15 damaged cells. Scale bars, 4 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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