FIGURE

Figure 7

ID

ZDB-FIG-190723-1490

Publication

Zhu et al., 2019 - Mutant Ahi1 Affects Retinal Axon Projection in Zebrafish via Toxic Gain of Function

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Figure 7

Ahi1 CRSIPR/Cas9-targeted embryos exhibited abnormal RGC axon projection patterns compared to WT embryos. (A) Fluorescent images of the optic nerve projection of WT and ahi1 CRSIPR/Cas9 targeted (ahi1mutant)-embryos at 4 dpf. Scale bar = 200 μm. (B) The statistical results of RGC axon length, abnormal crossing, and eye size of WT (n = 59) and ahi1 mutant (n = 46) embryos. (C) The ratios of embryos with normal retinal axon projection, abnormal middle crossing, and reduced axon elongation in WT and ahi1 mutant embryos. (D) The two-dimensional scatter diagram for the eye area of each WT or ahi1 mutant embryos. The X-axis represents the length of OT (μm²), and the Y-axis represents the size of eyes (μm²). Error bars denote SEM. ***p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR1-ahi1
Knockdown Reagent:	CRISPR1-ahi1
Observed In:	eye retinal ganglion cell axon retinal ganglion cell axon extension retinal ganglion cell axon guidance
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.