FIGURE

Figure 4

ID

ZDB-FIG-190723-1303

Publication

Marra et al., 2019 - Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

irx2a is required upstream of etv5a for MCC genesis. (A) Dorsal view of odf3b in the pronephros by WISH at the 28 ss shows a decrease in maturing MCCs in irx2a deficient embryos, which was rescued with irx2a cRNA, etv5a cRNA, and irx2a^sa10776 cRNA. Scale bar is 50 μm. (B) Quantification of odf3b⁺ MCCs at the 28 ss. Each dot represents one pronephros. Data is presented +/− SEM and statistical significance was determined with ANOVA. WISH – whole mount in situ hybridization, ss – somite stage, MCC – multiciliated cell.

Expression Data

Gene:	cimap1b
Fish:	TU TU + MO2-irx2a
Knockdown Reagent:	MO2-irx2a
Anatomical Term:	pronephric tubule multi-ciliated epithelial cell
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO2-irx2a
Knockdown Reagent:	MO2-irx2a
Observed In:	pronephric tubule pronephric tubule multi-ciliated epithelial cell pronephric tubule multi-ciliated epithelial cell differentiation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.