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A pipeline schematic of the hybrid method. a MS algorithm is applied to improve the visibility of the transparent regions and weak edges. b An enclosed contour is extracted from the segmentation candidate in (a). c A distance-regularised level set function (LSF) is initialised from the zebrafish contour in (b). d The ILS method is activated and applied on the original image. e Another segmentation candidate is generated. f An initial hybrid segmentation of the zebrafish is obtained by stitching the remarkable segments from the two candidates according to pre-defined protocols. g A refinement is followed to fine-tune the segmentation which can accurately represent the shape of the zebrafish
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