Figure 2

Proof-of-Principle. (A) Effect of DMSO concentration on HV assay. 1% DMSO vehicle has no effect on HV numbers. (B) The compounds of row D (D2-D11) and column 4 (4A-4H) in plate #30331 were tested individually and as pools. When 10 μM sunitinib was added to the pool “D” or “4” (pool D + Sun or pool4 + Sun), its antiangiogenic effects were as pronounced as if tested on its own (Sunitinib). (C) When compounds of plate #30251 were tested in pools, well H4 which was replaced with 10 μM sunitinib, was easily detected as antiangiogenic in both pools (“H” & “4”) containing H4. (D) A dose–response analysis with different concentrations of sunitinib alone or added to pool D in plate #30331 proves that weaker antiangiogenic effects can be reliably detected in pools. Shown are representative bright-field images of larvae, corresponding fluorescent images of dissected lenses and the quantification of primary hyaloid vessel numbers (right). Data shown are means ± SD. Statistical significance was calculated by t test and Mann Whitney test (*p < 0.05, **p < 0.01, *** p < 0.001).