ZFIN ID: ZDB-FIG-190716-1
Han et al., 2019 - Vitamin D Stimulates Cardiomyocyte Proliferation and Controls Organ Size and Regeneration in Zebrafish. Developmental Cell   48(6):853-863.e5 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Conditions:
Anatomical Term:
Stage Range: Day 4 to Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 4 to Adult

Fig. 1

 Vitamin D Analogs Promote Cardiomyocyte Proliferation in Zebrafish

(A) Cartoon schematic of in vivo chemical screen for regulators of CM proliferation using cmlc2:FUCCI zebrafish embryos. dpf, days post-fertilization.

(B) Maximum intensity projection (MIP) images of dissected cmlc2:FUCCI hearts. Veh, vehicle; v, ventricle; a, atrium. Scale bar, 50 μm.

(C) Quantification of FUCCI+ CMs after 24-h treatment. Mean ± SD, Mann-Whitney test and Student’s t test. ∗∗∗∗p < 0.0001. n = 11–13.

(D) Quantification of FUCCI+ CMs after 24-h treatment. Mean ± SD, Mann-Whitney test and Student’s t test. n = 7–10.

(E) Quantification of adult ventricular CM proliferation indices. Mean ± SD, Mann-Whitney test and Student’s t test. ns, not significant; ∗∗p < 0.01. n = 9–10.

(F) Representative images of PCNA/Mef2 staining quantified in (E). Boxed area is enlarged on the right. Arrowheads indicate PCNA+CMs. dpt, days post-treatment. Scale bar, 100 μm.

(G) Ventricular CM proliferation indices of vehicle- or Alfa-injected adult zebrafish. Mean ± SD, Mann-Whitney test and Student’s t test. n = 10–12.

(H) Representative images and quantification of ventricular PCNA/Mef2 staining. Mean ± SD, Mann-Whitney test. n = 9–10. Scale bar, 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry pd57Tg/+; pd58Tg/+ control Day 4 cardiac muscle cell IFL
pd57Tg/+; pd58Tg/+ chemical treatment by environment: alfacalcidol Day 4 cardiac muscle cell IFL
Venus pd57Tg/+; pd58Tg/+ control Day 4 cardiac muscle cell IFL
pd57Tg/+; pd58Tg/+ chemical treatment by environment: alfacalcidol Day 4 cardiac muscle cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mef2 IHC WT control Adult cardiac muscle cell
IHC WT chemical treatment by injection: alfacalcidol Adult cardiac muscle cell
IHC vdrapd307/pd307 ; vdrbpd308/pd308 chemical treatment by injection: alfacalcidol Adult cardiac muscle cell
Ab2-pcna IHC WT control Adult cardiac muscle cell
IHC WT chemical treatment by injection: alfacalcidol Adult cardiac muscle cell
IHC vdrapd307/pd307 ; vdrbpd308/pd308 chemical treatment by injection: alfacalcidol Adult cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by injection: alfacalcidol Adult cardiac ventricle cardiac muscle cell proliferation increased occurrence, abnormal
pd57Tg/+; pd58Tg/+ chemical treatment by environment: alfacalcidol Day 4 cardiac muscle cell proliferation increased occurrence, abnormal
pd57Tg/+; pd58Tg/+ chemical treatment by environment: calcipotriol Day 4 cardiac muscle cell proliferation increased occurrence, abnormal
vdrapd307/pd307 ; vdrbpd308/pd308 chemical treatment by injection: alfacalcidol Adult cardiac ventricle cardiac muscle cell proliferation occurrence, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 48(6), Han, Y., Chen, A., Umansky, K.B., Oonk, K.A., Choi, W.Y., Dickson, A.L., Ou, J., Cigliola, V., Yifa, O., Cao, J., Tornini, V.A., Cox, B.D., Tzahor, E., Poss, K.D., Vitamin D Stimulates Cardiomyocyte Proliferation and Controls Organ Size and Regeneration in Zebrafish, 853-863.e5, Copyright (2019) with permission from Elsevier. Full text @ Dev. Cell