FIGURE

Fig. 3

ID

ZDB-FIG-190712-17

Publication

Kim et al., 2019 - Distribution and neuronal circuit of spexin 1/2 neurons in the zebrafish CNS

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Fig. 3

spx 1 and spx 2 expression in the hypothalamic area of zebrafish larvae. (A–E) Labelling of 7 dpf Tg(spx1:mCherry-CAAX) (A–C), Tg(spx1:gal4; uas:egfp) (D), and wildtype larvae (E) with antibodies against α-melanocyte-stimulating hormone (α-MSH) (A,D), agouti-related peptide (AgRP) (B), corticotropin-releasing hormone (CRH) (C), and corticotropin releasing hormone b(crhb) RNA probe (E). Ventral views with anterior to the left. White arrowheads indicate α-MSH expression in the pituitary gland (A,D), and white arrows label AgRP-expressing neurons in the ventral hypothalamus (B) and CRH⁺ axons in the hypothalamus (C). (E) Expression of spx2 and crhb mRNA in the larval brain revealed by in situ RNA hybridization. Yellow arrow designates neurons co-expressing spx2 and crhb mRNA in the preoptic region of the hypothalamus. Scale bar: 25 μm.

Expression Data

Genes:	crhb EGFP mCherry tesmin
Antibodies:	Ab1-agrp Ab1-aMSH Ab1-crh
Fish:	AB km8Tg km9Tg; nkuasgfp1aTg
Anatomical Terms:	hypophysis hypothalamus periventricular nucleus preoptic area
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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