ZFIN ID: ZDB-FIG-190606-41
Pickett et al., 2018 - Cumulative mitochondrial activity correlates with ototoxin susceptibility in zebrafish mechanosensory hair cells. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Fish:
Condition:
Anatomical Terms:
Stage: Day 5

Fig. 5-S1

Reduced hair cell activity does not influence hair cell turnover.

Maximum projects of (A) DMSO control and (B) benzamil-treated fish expressing hair cell-specific membrane GFP (Tg[Pou4f3:gap43-GFP]) and photoconverted, nuclear localized EOS (Tg[Atoh1a:nls-Eos]w214) at 5dpf. (C) The ratio of red label-retaining hair cells to total number of hair cells per neuromast counted at 5dpf. There is no difference between control and treated groups, indicating that reduced MET does not lead to increased hair cell turnover. Control: 0.72 ± 0.12, n = 8 control fish; Treated: 0.70 ± 0.14, mean ratio ±SD, n = 10 fish; 2–3 neuromasts analyzed per fish; Mann-Whitney U test, p = 0.76. Asterisks indicate hair cells with label-retaining nuclei. Scale bar = 5 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Eos atoh1aw214Tg; s356tTg control Day 5 neuromast hair cell IFL
Day 5 neuromast support cell IFL
atoh1aw214Tg; s356tTg chemical treatment: Benzamil Day 5 neuromast hair cell IFL
Day 5 neuromast support cell IFL
GFP atoh1aw214Tg; s356tTg control Day 5 neuromast hair cell membrane IFL
atoh1aw214Tg; s356tTg chemical treatment: Benzamil Day 5 neuromast hair cell membrane IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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