FIGURE

Fig. 4

ID

ZDB-FIG-190604-53

Publication

Lan et al., 2019 - TETs Regulate Proepicardial Cell Migration through Extracellular Matrix Organization during Zebrafish Cardiogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

AVC Development Shows Disruption in tet2/3^DMLarvae

(A) GFP-labeled AVC endocardium represents Wnt activity in sibling heart, but not tet2/3^DM heart. Hearts were dissected from 48-hpf larvae carrying the Tg(7xTCF-Xla.Siam:GFP) transgene. White arrows indicate AVC endocardial cells with Wnt activity.

(B) WISH for AVC markers bmp4 and has2 at 48 hpf.

(C) GFP-labeled endocardium represents AV valve formation in 4-dpf sibling, but not tet2/3^DM larvae carrying the Tg(kdrl:EGFP) transgene. White arrows indicate the AV valve. Bottom left images show higher magnification views of AV valve regions.

(D) WISH for AVC marker bmp4 in 48 hpf and confocal imaging for GFP-labeled PE and epicardium in 72-hpf control and IWR-1-treated larvae.

Scale bars: 50 μm.

Expression Data

Genes:	bmp4 GFP has2
Fish:	WT ia4Tg tet2^mk17/mk17; tet3^mk18/mk18 tet2^mk17/mk17; tet3^mk18/mk18; ia4Tg
Anatomical Terms:	atrioventricular canal atrioventricular canal endocardium
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tet2^mk17/mk17; tet3^mk18/mk18 tet2^mk17/mk17; tet3^mk18/mk18; ia4Tg tet2^mk17/mk17; tet3^mk18/mk18; ubs1Tg
Observed In:	atrioventricular canal atrioventricular canal endocardium atrioventricular valve Wnt signaling pathway involved in heart development
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.