FIGURE

Fig. 6

ID

ZDB-FIG-190530-17

Publication

Konjikusic et al., 2018 - Mutations in Kinesin family member 6 reveal specific role in ependymal cell ciliogenesis and human neurological development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

(A-D) Immunofluorescence of Tg[foxj1a::GFP] (A, B) and Tg[foxj1a::Arl13bGFP] transgenic zebrafish in both heterozygous kif6sko/+ (A, C) and homozyogus kif6sko mutant (B, D) zebrafish backgrounds assayed with ?GFP (green) and DAPI (nuclei, blue). The Tg[foxj1a::GFP] transgene demonstrates that GFP positive ECs are present in both genotypes. In contrast, kif6sko/+ heterozygous zebrafish display numerous apical tufts of cilia (red arrows; A) projecting into the ventricle lumen, which are markedly reduced in kif6sko mutant zebrafish (B). Similar results were observed with the Tg[foxj1a::Arl13bGFP] transgene, showing a obvious reduction in Arl13b-GFP positive EC axonemes in kif6sko mutant zebrafish (D), which are robustly labeled in kif6sko/+ heterozygous fish (C). Scale Bars: 20?m

Expression Data

Gene:	GFP
Fish:	kif6^gw326/+; ut1Tg kif6^gw326/gw326; ut1Tg kif6^gw326/+; ut22Tg kif6^gw326/gw326; ut22Tg
Anatomical Term:	ependymal cell cilium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	kif6^gw326/gw326; ut1Tg kif6^gw326/gw326; ut22Tg
Observed In:	ependymal cell axoneme ependymal cell cilium
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.