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ZIRC
ZFIN ID: ZDB-FIG-190524-22
Rasouli et al., 2018 - The flow responsive transcription factor Klf2 is required for myocardial wall integrity by modulating Fgf signaling. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 2

Cardiac contractility is required for cardiomyocyte extrusion.

(A) Maximum intensity projections of confocal z-stacks of 96 hpf klf2 WT and mutant hearts, non-injected or injected with tnnt2a MO at the one-cell stage; part of ventricular outer curvature (dashed boxes) magnified on the right side of each panel. (B) Time-lapse 2D confocal images of a klf2 mutant heart during BDM treatment. Arrows point to an extruding cardiomyocyte returning to the compact layer upon inhibiting contraction; V: ventricle; At: atrium; scale bars: 50 µm (A), 10 µm (B).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRedx hsc4Tg ; s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 standard conditions Day 4 cardiac muscle cell nucleus IFL
Day 4 ventricular myocardium cardiac muscle cell IFL
hsc4Tg ; s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 chemical treatment by environment: Diacetylmonoxime Day 4 cardiac muscle cell nucleus IFL
Day 4 ventricular myocardium cardiac muscle cell IFL
GFP hsc4Tg ; s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 standard conditions Day 4 ventricular myocardium cardiac muscle cell IFL
hsc4Tg ; s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 chemical treatment by environment: Diacetylmonoxime Day 4 ventricular myocardium cardiac muscle cell IFL
s974Tg standard conditions Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
Day 4 ventricular myocardium cardiac muscle cell IFL
s974Tg + MO1-tnnt2a standard conditions Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
Day 4 ventricular myocardium cardiac muscle cell IFL
s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 standard conditions Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
Day 4 ventricular myocardium cardiac muscle cell IFL
s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 + MO1-tnnt2a standard conditions Day 4 atrial myocardium IFL
Day 4 ventricular myocardium IFL
Day 4 ventricular myocardium cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hsc4Tg ; s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 standard conditions Day 4 cardiac muscle cell protruding out of compact layer of ventricle, abnormal
Day 4 ventricular myocardium ventricular cardiac muscle tissue morphogenesis decreased process quality, abnormal
hsc4Tg ; s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 chemical treatment by environment: Diacetylmonoxime Day 4 atrium cardiac muscle contraction arrested, abnormal
Day 4 cardiac muscle cell located in compact layer of ventricle, ameliorated
Day 4 cardiac ventricle cardiac muscle contraction arrested, abnormal
Day 4 ventricular myocardium ventricular cardiac muscle tissue morphogenesis process quality, ameliorated
s974Tg + MO1-tnnt2a standard conditions Day 4 atrium cardiac muscle contraction arrested, abnormal
Day 4 cardiac muscle cell located in compact layer of ventricle, normal
Day 4 cardiac ventricle cardiac muscle contraction arrested, abnormal
s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 standard conditions Day 4 cardiac muscle cell protruding out of compact layer of ventricle, abnormal
Day 4 ventricular myocardium ventricular cardiac muscle tissue morphogenesis decreased process quality, abnormal
s974Tg ; klf2abns11/bns11 ; klf2bbns12/bns12 + MO1-tnnt2a standard conditions Day 4 atrium cardiac muscle contraction arrested, abnormal
Day 4 cardiac muscle cell located in compact layer of ventricle, ameliorated
Day 4 cardiac ventricle cardiac muscle contraction arrested, abnormal
Day 4 ventricular myocardium ventricular cardiac muscle tissue morphogenesis process quality, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife