Fig. 4

Localization of biotransformation of benzyloxy-methyl-resorufin (BOMR) in the trunk region of intact zebrafish embryos and larvae at 26 h post-fertilization (hpf) (b,c), 50 hpf (d,e), 74 hpf (f,g), 98 hpf (h,i), 122 hpf (j,k), 9 d post-fertilization (dpf) (l,m) and 14 dpf (n,o). At 7 hpf (a), qualitative analysis of resorufin formation was performed in the whole embryo. Pictures show one embryo/larva out of six used in the study, i.e., three biological replicates with two embryos/larvae per replicate, for each developmental stage. Figure 4a shows a vegetal pole view of the embryo. In Figure 4b?o lateral views of the anterior and posterior part of the trunk region are shown. The organs in which resorufin had been formed are indicated with a two-letter combination. Since the otic vesicle is part of the head region, resorufin formation in the respective organ is mentioned separately. S.B.: swim bladder. Scale bar: 200 µm; anterior left and dorsal top.