The lethality of the slc12a10.2-deficient zebrafish is rescued by exogenous Cl− supplement. (A) Schematic diagram of the rearing timeline. Larvae were reared in egg water from 0 to 5 dpf, and in system water after 5 dpf. The survival ratio of fish reared under this condition was analyzed until 20 dpf. (B) Comparison of the survival ratios of prl+/+, prl−/−, slc12a10.2+/+, and slc12a10.2−/− larvae (n = 25 for each genotype). (C–H) The general morphological observations of control larvae and slc12a10.2-deficient larvae at 6, 8, and 10 dpf. (C,E,G) Control larvae. (D,F,H) slc12a10.2-deficient larvae. (I) Schematic diagram of the rearing timeline. Larvae were reared in egg water from 0 to 5 dpf and in brackish or artificial water after 5 dpf. The survival ratios of fish reared under different conditions were analyzed until 20 dpf. (J–M) The survival ratios of control and slc12a10.2-deficient larvae in system water + sea salt, system water + Mix 1, system water + Mix 2, and system water + Mix 3.
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