FIGURE

Fig. 1

ID

ZDB-FIG-190322-7

Publication

Shu et al., 2018 - Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Generation of slc12a10.2-deficient zebrafish. (A) WISH analysis using the slc12a10.2 probe in wild-type larvae at 4 dpf. (B) Targeted depletion of the slc12a10.2 gene. The CRISPR/Cas9 target site is located at exon 1. A genotype with an 11-bp deletion was used to establish the slc12a10.2 knockout line (highlighted in red). (C) Schematic diagram of the rearing timeline. Larvae were reared in egg water from 0 to 5 dpf and in system water after 5 dpf. The fish at 6 dpf were harvested for Na+ and Cl− measurements. (D,E) Na+ and Cl− concentrations were measured in control and slc12a10.2-deficient larvae at 6 dpf. **P < 0.01. (F,G) Sodium green staining in the gills of control and slc12a10.2-deficient larvae at 6 dpf (ventral views).

Expression Data

Gene:	slc12a10.2
Fish:	AB
Anatomical Terms:	gill integument
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc12a10.2^{ihb342/ihb342}
Observed In:	gill sodium ion transport whole organism chloride whole organism sodium(1+)
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)