FIGURE

Fig. 3

ID

ZDB-FIG-190131-23

Publication

Kustermann et al., 2018 - Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo

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Fig. 3

Inactivation of Vcp leads to an accumulation of ubiquitinated proteins and Sqstm1. (a) Western blot analysis using an anti-K48-linkage specific ubiquitin antibody after MO1-control and MO1-vcp injection and DMSO or MG132 treatment (n = 4). (b) Vcp morphants show a significant increase of Sqstm1 by western blot analysis (n = 3); quantification of gray values. Actb1/β-actin was used as loading control. Data represent means ± SD, unpaired Student t test, *P value< 0.03 (c) Western blot analysis of Lc3 after MO1-control and MO1-vcp injection and DMSO or Ammonium chloride (NH4Cl) treatment (top) and quantification of gray values (bottom). NH4Cl treated control embryos reveal significant increase of Lc3-II levels, compared to DMSO treated embryos (n = 5). Actb1/β-actin was used as loading control. The individual samples are displayed (two-sided Wilcoxon rank-sum test).

Expression Data

Antibodies:	Ab1-map1lc3 Ab4-ub Ab7-sqstm1
Fish:	WT WT + MO4-vcp
Conditions:	chemical treatment by environment: N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal chemical treatment by environment: ammonium chloride
Knockdown Reagent:	MO4-vcp
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO4-vcp
Conditions:	chemical treatment by environment: N-benzyloxycarbonyl-L-leucyl-L-leucyl-L-leucinal chemical treatment by environment: ammonium chloride
Knockdown Reagent:	MO4-vcp
Observed In:	autophagy proteasome-mediated ubiquitin-dependent protein catabolic process whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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