Fig. S5

Histological analysis of the intestines does not show structural defects in irf8 mutants, Related to Figure 4.

A Schematic of the adult zebrafish intestine and corresponding proximal (S1-S4) and distal (S5-S7) regions. B Schematic of quantified parameters for assessment of intestinal architecture: gut wall thickness, villus fold height, and frequency of goblet cells. C Representative images of proximal (left) and distal (right) intestinal regions stained with combined Alcian Blue and Periodic Acid-Schiff on 5 um transverse sections of irf8 wild type animals (top) and mutant animals (bottom). D Quantification of intestinal wall thickness (left), number of goblet cells per intestinal villus fold (middle) and intestinal villus fold height (right). Data were derived from n= 3 wild type siblings for proximal and distal regions, n=4 mutants for proximal region, and n=5 mutants for distal region. All scale bars represent 100 μm. Statistical significance was determined by the Mann-Whitney test.