FIGURE

Fig. S4

ID

ZDB-FIG-181116-35

Publication

Koch et al., 2018 - Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88

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Fig. S4

(A) Image illustrating the separation of intestinal tissues from body tissues, prior to RNA isolation and qPCR analysis of intestinal versus body (B) q-PCR analysis comparing the relative expression levels of cebpb, fosl1a and il1b, in conventionalized (CONVD) versus germ-free (GF) conditions in samples derived from excised intestinal bulp and in the remaining body tissues, (mean ± s.e.m, n=3 biological replicates, 30 embryos per group) **p0,01; ****p0,0001 by Student’s t-test (C) Wholemount in sity hybridization results of cebpb and fosl1a, scale bar represents 1 mm (D) Representative images of germ-free and conventionalized larvae in the Tg(il1b:GFP) reporter line stitched from confocal stacks acquired with 10x magnication scalebars, 500 μm in A and D; 1 mm in C.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.