PUBLICATION
Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88
- Authors
- Koch, B.E.V., Yang, S., Lamers, G., Stougaard, J., Spaink, H.P.
- ID
- ZDB-PUB-181007-3
- Date
- 2018
- Source
- Nature communications 9: 4099 (Journal)
- Registered Authors
- Koch, Bjorn, Spaink, Herman P.
- Keywords
- none
- Datasets
- GEO:GSE82200
- MeSH Terms
-
- Animals
- Gastrointestinal Microbiome/immunology*
- Genes, Reporter
- Immunity, Innate*
- Intestinal Mucosa/immunology*
- Myeloid Differentiation Factor 88/genetics
- Myeloid Differentiation Factor 88/metabolism*
- Toll-Like Receptor 2/metabolism
- Transcriptome
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 30291253 Full text @ Nat. Commun.
Citation
Koch, B.E.V., Yang, S., Lamers, G., Stougaard, J., Spaink, H.P. (2018) Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88. Nature communications. 9:4099.
Abstract
Host pathways mediating changes in immune states elicited by intestinal microbial colonization are incompletely characterized. Here we describe alterations of the host immune state induced by colonization of germ-free zebrafish larvae with an intestinal microbial community or single bacterial species. We show that microbiota-induced changes in intestinal leukocyte subsets and whole-body host gene expression are dependent on the innate immune adaptor gene myd88. Similar patterns of gene expression are elicited by colonization with conventional microbiome, as well as mono-colonization with two different zebrafish commensal bacterial strains. By studying loss-of-function myd88 mutants, we find that colonization suppresses Myd88 at the mRNA level. Tlr2 is essential for microbiota-induced effects on myd88 transcription and intestinal immune cell composition.
Errata / Notes
This article is corrected by ZDB-PUB-220906-137 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping