Fig. 1

Forskolin treatment reduces iridophore number in zebrafish larvae at 4 days post fertilization (dpf). (A,B) Lateral images (using an incident lighting source) of larvae at 4 dpf treated with 0.1% dimethyl sulfoxide (DMSO) or 5 µM forskolin in embryo media. Forskolin-treated individuals have fewer iridophores, especially in the ventral stripe (arrowheads). Scale bar = 200 um and applies to panels A and BC) Quantification of total, dorsal stripe, and ventral stripe iridophores (n = 8–10 larvae per condition). Forskolin treatment leads to a 38.4% reduction in differentiated iridophores (62.6% of control number). (D) Quantification of melanophores following 0.1% DMSO or 5 µM forskolin treatment. Total melanophores include dorsal, lateral, and ventral stripes (n = 8–10 larvae per condition), showing no significant change following forskolin treatment.