ZFIN ID: ZDB-FIG-181030-19
Jokl et al., 2018 - Transcriptional upregulation of Bag3, a chaperone-assisted selective autophagy factor, in animal models of KY-deficient hereditary myopathy.. Disease models & mechanisms   11(7) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5

Fig. 5

Methylcellulose challenge does not increase transcriptional upregulation of CASA in kyyo1/kyyo1 embryos. (A) qPCR of bag3, flnca and flncb shows significantly increased transcription in 5 dpf kyyo1/kyyo1 embryos compared with WT controls when grown in normal (E3) media (one-way ANOVA/Dunnett's; *P<0.05, **P<0.01, n=3). Swimming in media enriched with 1% methylcellulose (Me) from 3 dpf to 5 dpf induces significant increases in bag3 and flnca transcription and reveals a trend towards increased flncb transcription in WT embryos (one-way ANOVA/Dunnett's; *P<0.05, **P<0.01, n=3). No significant changes in transcription are observed between challenged and unchallenged kyyo1/kyyo1 embryos. Error bars indicate s.e.m. (B) Fibre-typing of embryonic zebrafish muscle shows no changes to slow (green) or fast (red) muscle distribution. Images are representative of five WT and four kyyo1/kyyo1 embryos per treatment group (see Fig. S4 for full panel). (C) Representative images of birefringence analysis of whole zebrafish embryos, showing no gross muscle damage or deformation in any treatment group. These observations indicate that transcriptional changes are not secondary to gross muscle damage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bag3 WT chemical treatment by environment: methyl cellulose Day 5 whole organism RTPCR
kyyo1/yo1 chemical treatment by environment: methyl cellulose Day 5 whole organism RTPCR
flnca WT chemical treatment by environment: methyl cellulose Day 5 whole organism RTPCR
kyyo1/yo1 chemical treatment by environment: methyl cellulose Day 5 whole organism RTPCR
flncb WT chemical treatment by environment: methyl cellulose Day 5 whole organism RTPCR
kyyo1/yo1 chemical treatment by environment: methyl cellulose Day 5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: methyl cellulose Day 5 fast muscle cell spatial pattern, normal
Day 5 slow muscle cell spatial pattern, normal
Day 5 whole organism bag3 expression increased amount, abnormal
Day 5 whole organism flnca expression increased amount, abnormal
Day 5 whole organism flncb expression increased amount, abnormal
Day 5 whole organism refractivity, normal
kyyo1/yo1 standard conditions Day 5 fast muscle cell spatial pattern, normal
Day 5 slow muscle cell spatial pattern, normal
Day 5 whole organism refractivity, normal
kyyo1/yo1 chemical treatment by environment: methyl cellulose Day 5 fast muscle cell spatial pattern, normal
Day 5 response to mechanical stimulus process quality, abnormal
Day 5 slow muscle cell spatial pattern, normal
Day 5 whole organism bag3 expression increased amount, abnormal
Day 5 whole organism flnca expression increased amount, abnormal
Day 5 whole organism flncb expression increased amount, abnormal
Day 5 whole organism refractivity, normal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.