FIGURE

Fig. 1

ID

ZDB-FIG-180928-2

Publication

Tiefenbach et al., 2018 - Idebenone and coenzyme Q₁₀ are novel PPARα/γ ligands, with potential for treatment of fatty liver diseases.

Other Figures

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Fig. 1

PPAR LT fish. (A) In the ligand trap (LT) system (Chawla et al., 1994), a DNA-binding domain (DBD)?human nuclear receptor ligand-binding domain (LBD) fusion protein is used to signal the presence of ligand in vivo. In the presence of specific ligands and cofactors, binding of the fusion protein to a GFP reporter results in expression of GFP. Previously published by Tiefenbach et al. (2010) under a CC-BY license (https://creativecommons.org/licenses/by/4.0/). (B) 2 dpf PPAR?, PPAR? and PPAR? LT fish respond to endogenous fish hormones and PPAR-specific agonists. Active concentrations were similar to those observed in human cells, and non-specific cross-receptor activities were not observed for any of the tested agonists on the other two PPAR receptors (mean n=5).

Expression Data

Gene:	EGFP
Fish:	zf225Tg zf2021Tg zf2022Tg
Conditions:	chemical treatment by environment: GW 0742 chemical treatment by environment: rosiglitazone chemical treatment by environment: pirinixic acid
Anatomical Terms:	blood brain central nervous system epidermis forebrain heart keratinocyte retina skeletal muscle spinal cord posterior region
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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