ZFIN Image: Tiefenbach et al., 2018, Fig. 1

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Figure Caption

Fig. 1

PPAR LT fish. (A) In the ligand trap (LT) system (Chawla et al., 1994), a DNA-binding domain (DBD)–human nuclear receptor ligand-binding domain (LBD) fusion protein is used to signal the presence of ligand in vivo. In the presence of specific ligands and cofactors, binding of the fusion protein to a GFP reporter results in expression of GFP. Previously published by Tiefenbach et al. (2010) under a CC-BY license (https://creativecommons.org/licenses/by/4.0/). (B) 2 dpf PPARα, PPARδ and PPARγ LT fish respond to endogenous fish hormones and PPAR-specific agonists. Active concentrations were similar to those observed in human cells, and non-specific cross-receptor activities were not observed for any of the tested agonists on the other two PPAR receptors (mean n=5).

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Expression / Labeling details

Acknowledgments

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