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ZFIN ID: ZDB-FIG-180927-21
Peters et al., 2018 - Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish.. Biology Open   7(8) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Adult

Fig. 6

Promoter elements have distinct roles in driving hematopoietic, renal and testicular expression of Hemogen in transgenic Tg(Hemgn:mCherry) zebrafish. (A) Schematic of the zebrafish Hemogen gene. CNEs, black; coding exons, white; transcription initiation sites, bent arrows. Three Tg(Hemgn:mCherry,myl7:EGFP) transgenes driven by portions of the Hemogen promoter were transfected into one-cell TU embryos by Tol2 transposase-mediated insertion. Numbers indicate length of promoter elements and arrows show gene direction. (B) 20 hpf. P1 transgene expression in the peripheral blood island (PBI). (C) 72 hpf. P1 transgene expression in the pronephric ducts (PD). (D) 5 dpf. P1 transgene expression in the proximal convoluted tubule (PCT). (E,F) 72 hpf. Co-localization of mCherry and EGFP in progenitors in the CHT of Tg(Hemgn-P1:mCherry,Lcr:GFP) or Tg(Hemgn-P1:mCherry,CD41:EGFP) zebrafish. (G) Transgene expression in mature erythrocytes from adult zebrafish. (H) Transgene expression in adult head kidney (HK), trunk kidney (TK) and tail kidney (T) near the EGFP+ heart (H). (I) Transgene expression in adult Sertoli cells (Se) that surround the seminiferous tubules (ST). (J) Proportion of embryos expressing transgenes P1, P2 or P3 in ICM, kidney, CHT and circulating primitive erythrocytes (RBC). Scale bars: 100 µm (B,D-F,I); 500 µm (C,H); 25 µm (G).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry cz3325Tg ; nuz1Tg standard conditions Protruding-mouth caudal hematopoietic tissue erythroid progenitor cell IFL
Protruding-mouth Not Detected thymus IFL
Protruding-mouth Not Detected ventral wall of dorsal aorta IFL
la2Tg ; nuz1Tg standard conditions Protruding-mouth caudal hematopoietic tissue common myeloid progenitor IFL
Protruding-mouth caudal hematopoietic tissue erythroid progenitor cell IFL
Protruding-mouth Not Detected thymus IFL
Protruding-mouth Not Detected ventral wall of dorsal aorta IFL
nuz1Tg standard conditions 20-25 somites intermediate cell mass of mesoderm IFL
Protruding-mouth pronephric duct IFL
Day 5 pronephric proximal convoluted tubule IFL
Adult head kidney nucleate erythrocyte IFL
Adult Sertoli cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open