FIGURE

Fig. 1

ID
ZDB-FIG-180917-34
Publication
Nichols et al., 2018 - Ensheathing cells utilize dynamic tiling of neuronal somas in development and injury as early as neuronal differentiation
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Sensory neurons become ensheathed shortly after neuronal differentiation. (a). Confocal z-projection frame of a Tg(sox10:mcherry); Tg(ngn1:gfp) zebrafish DRG at 72 hpf showing complete ensheathment. (b). Diagram of sensory neuron cell soma ensheathment by satellite glia. (c). Diagram of Eos photoconversion paradigm before neuronal differentiation. (d). Confocal z-projection images from a 24-h timelapse starting at 48 hpf of a Tg(sox10:eos) zebrafish with a photoconverted DRG neuron showing ensheathment of neuronal cell soma. White arrows denote dynamic projections of the ensheathing cell circumnavigating the neuron soma. (e). Confocal three-dimensional images of a nascent Tg(sox10:eos) DRG with a photoconverted neuron before and after ensheathment. D denotes dorsal, M denotes medial, V denotes ventral, and L denotes lateral. (f). Plot of two ensheathing processes converging on the same area of the of the neuron. (0,0) represents the site of the convergence of ensheathing processes. Red circle denotes the location of the neuron cell soma. (g). Intensity profiles transecting two approaching ensheathing processes every 75 min. (h). Deconvolved confocal z-projection images of two approaching ensheathing projections represented in (g). White brackets denote the gap between the two ensheathing processes. White arrows denote the emergence of the cellular processes. (i, j). Graphs of the areas of neurons (i) and ensheathing cells (j) before and after neuronal soma ensheathment (n = 5 DRG). (i, j) use a paired Student’s t-test. Scale bar is 10 μm (a, d, e, h). All intensity measurements were taken from sum z-projections

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.