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ES supplementation rescues pigmentation and the Cox1 deficiency of ctr1-KO zebrafish. (A–C) Clutches of embryos from a WT zebrafish and from a cross of a pair of ctr1+/− heterozygous zebrafish were imaged at 48 hpf following treatment with and without 10 nM ES. Arrows in B indicate homozygous ctr1−/− embryos with a pigmentation defect. (D) Mitochondria were isolated from 10-dpf WT zebrafish and homozygous ctr1−/− zebrafish treated with and without 10 nM ES. Mitochondrial samples were subjected to SDS/PAGE and immunoblotted using the Complex IV-specific antibody anti-Cox1. The mitochondrial protein Atp5a was used as a loading control.
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