FIGURE

Fig. 2

ID

ZDB-FIG-180913-10

Publication

Thwaite et al., 2018 - Protein Nanoparticles Made of Recombinant Viral Antigens: A Promising Biomaterial for Oral Delivery of Fish Prophylactics

Other Figures

Fig. 2

All Figure Page

Back to All Figure Page

Fig. 2

Uptake of viral nanopellets (NPs) by ZFL. Fluorescently labeled NPs (A) IPNV-VP2^NP, (B) VHSV-G-frg16^NP, and (C) VNNV-C^NP were added to ZFL. Control (ctl) was ZFL without NPs. (i) Dose–response. Cells incubated for 12 h with NPs (A,C) at 5–20 µg/ml, and (B) at 1–20 µg/ml in duplicate. (ii) Time course. NPs added to cells at 10 µg/ml (A,C), and 5 µg/ml (B) in duplicate and incubated for 6–48 h. Differences between means were analyzed by a one-way ANOVA with Dunnett’s multiple comparisons test, treatments versus control. Significance levels *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (iii) Confocal microscopy and digitalized image (z-stacks) of ZFL cells after 14 h incubation with 20 µg/ml (A,C) and 10 µg/ml (B). NPs are green, cell membrane red, and nuclei blue. Control confocal image ZFL without NPs (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol