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Fig. 1

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ZDB-FIG-180912-34

Publication

Morris et al., 2018 - Live imaging of collagen deposition during skin development and repair in a collagen I - GFP fusion transgenic zebrafish line

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Fig. 1

Generation of a GFP labelled collagen I zebrafish line. (A) The N-terminal regions of zebrafish, mouse and human collagen I ?2 chains were aligned to determine the N-terminal proteinase cleavage site (red line) and identify the optimal GFP insertion site. (B) By inserting GFP in place of the N-terminal pro- and telo- peptide and removing the N-terminal proteinase site, GFP was retained on the ?2 monomer. (C) GFP-tagged ?2 trimerises with unlabelled ?1a and ?1b monomers and fibrillogenesis occurs with labelled and unlabelled trimers. (D) Tg(krt19:col1a2?GFP) transgenic fish exhibit GFP labelling within flank skin when compared to control, non-transgenic zebrafish where only the gut shows faint autofluorescence. D is composed of a 4-image tilescan confocal image of a 4?dpf zebrafish. Scale bar?=?0.5?mm.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 441(1), Morris, J.L., Cross, S.J., Lu, Y., Kadler, K.E., Lu, Y., Dallas, S.L., Martin, P., Live imaging of collagen deposition during skin development and repair in a collagen I - GFP fusion transgenic zebrafish line, 4-11, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.