ZFIN Image: Morris et al., 2018, Fig. 1

Image

Figure Caption

Fig. 1

Generation of a GFP labelled collagen I zebrafish line. (A) The N-terminal regions of zebrafish, mouse and human collagen I α2 chains were aligned to determine the N-terminal proteinase cleavage site (red line) and identify the optimal GFP insertion site. (B) By inserting GFP in place of the N-terminal pro- and telo- peptide and removing the N-terminal proteinase site, GFP was retained on the α2 monomer. (C) GFP-tagged α2 trimerises with unlabelled α1a and α1b monomers and fibrillogenesis occurs with labelled and unlabelled trimers. (D) Tg(krt19:col1a2‐GFP) transgenic fish exhibit GFP labelling within flank skin when compared to control, non-transgenic zebrafish where only the gut shows faint autofluorescence. D is composed of a 4-image tilescan confocal image of a 4 dpf zebrafish. Scale bar = 0.5 mm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 441(1), Morris, J.L., Cross, S.J., Lu, Y., Kadler, K.E., Lu, Y., Dallas, S.L., Martin, P., Live imaging of collagen deposition during skin development and repair in a collagen I - GFP fusion transgenic zebrafish line, 4-11, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.