Fig. 6

(a?c) Eye fluorescence assay: peak fluorescence intensity in the retinal vascular bed of ctns^?/? zebrafish larvae and wild-type larvae (N?=?20 each). Fluorescence intensities were evaluated using fixed diameter circles by the ImageJ software. (a) A representative wild-type 4 dpf larva (24?h post-injection) (bar?=?200??m). (b) A representative ctns^?/? 4 pdf larva (24?h post-injection) (bar?=?200??m). (c) Quantitation of peak fluorescence intensities in the retinal vascular bed of both genotypes. (d?i) Histopathological functional evaluation: (d) A representative proximal tubule of wt larva injected with the 70-kDa labelled dextran (bar?=?10??m). (e) A representative proximal tubule of wt larva injected with the 4-kDa labelled dextran (bar?=?10??m). (f) A representative proximal tubule of ctns^?/? larva injected with the 70-kDa labelled dextran (bar?=?10??m). (g) A representative proximal tubule of ctns^?/? larva injected with the 4-kDa labelled dextran (bar?=?10??m). (h) A higher magnification of the proximal tubules of both genotypes showing internalized 70-kDa dextran within cytosolic puncta that likely correspond to endocytic compartments (marked areas in panels d and f) (bars?=?5??m). (i) Quantitation of the number of dextran puncta in both high and low molecular weight dextran injections in both genotypes (N?=?10 for each genotype and each condition). *P?<?0.05, ***P?<?0.001.