Fig. 5

(a) H&E stained cut-section of a 6?dpf wt larva at the level of the glomerulus and proximal tubules (bar?=?50??m). (b) H&E stained cut-section of a 6 dpf ctns^?/? larva at the level of the glomerulus and proximal tubules showing no apparent abnormality (bar?=?50??m). (c) Block face scanning EM image of the proximal tubule of a 4?dpf wt larva (bar?=?5??m). Demarcated area was magnified (right) to show size and distribution of lysosomes (asterisks) in the wt (bar?=?2??m). (d) Block face scanning EM image of the proximal tubule of a 4?dpf ctns^?/? larva showing intact brush border (bar?=?5??m). Demarcated area was magnified (right) to show larger number of lysosomes (asterisks) many of which were significantly enlarged in size compared to the wt (bar?=?2??m). (e) Quantitation of the number and surface area of lysosomes in cut sections at the level of proximal tubules in both genotypes. (f) Transmission EM image of the glomerulus of a 6?dpf wt larva showing normal foot processes (bar?=?2??m). A magnified EM image (right) of podocytes of 6 dpf wt larva showing preserved podocytes slit diaphragms (bar?=?1??m). (g) Transmission EM image of the glomerulus of a 6 dpf ctns^?/? larva showing partial foot process effacement (black arrows) (bar?=?2??m). A magnified EM image (right) of podocytes of 6 dpf ctns^?/? larva showing narrowed podocyte slit diaphragmatic spaces (white arrows) (bar?=?1??m). (h) Quantitation of podocyte foot process width (FPW) in cut sections at the level of the glomerulus in both genotypes. bb, brush border; bs, Bowman?s space; g, glomerulus; n, nucleus; pt, proximal tubule. *P?<?0.05, ***P?<?0.001 between the 2 genotypes using student?s t test.