FIGURE

Fig. 3

ID

ZDB-FIG-180905-32

Publication

Akle et al., 2017 - Circadian Kinetics of Cell Cycle Progression in Adult Neurogenic Niches of a Diurnal Vertebrate

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Fig. 3

Nighttime transition of dividing cells through G₂/M phases of the cell cycle in the cerebellum of adult zebrafish. A–C, Number of cells expressing pH3, a late G₂ and early M phase marker, and BrdU, a marker for S phase. A, Cells positive for pH3-only increase throughout the night, B, Cells with colocalized BrdU/pH3 label decline throughout the night. n = 3–6 fish per time point; mean ± SEM; *p < 0.05 relative to the trough in labeled cells (pH3-only, ZT11; BrdU/pH3, ZT23). The gray background indicates night, 14:10 LD cycle. Fish were collected at specified ZT, after a 2 h in vivo exposure to BrdU. ZT14 is lights-off time. C, Representative confocal image: cells labeled for pH3-only (red), BrdU-only (green), and BrdU/pH3 merge (yellow). Scale bar, 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.