FIGURE

Fig. 4

ID

ZDB-FIG-180828-16

Publication

Tan et al., 2018 - Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish

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Fig. 4

Amelioration of MLL-AF9-induced hematopoiesis by MI-2. The menin inhibitor MI-2 (2.5mmol/L) and DMSO (control) were administered to MLL-AF9-injected and wild-type embryos from 24 to 72hpf. WISH assays of the (a) definitive hematopoietic stem cell marker c-myb and (b–d) myeloid lineage markers mpo, lyz, and l-plastin in wild-type embryos treated with DMSO (top), MLL-AF9-injected embryos treated with DMSO (middle), and MLL-AF9-injected embryos treated with MI-2 (bottom) at 72hpf. (e) Relative expression of the marker genes for definitive hematopoiesis (runx1 and c-myb) and myeloid hematopoiesis (l-plastin, csf1r, mfap4, mpo, and lyz) in wild-type and MLL-AF9-injected embryos treated with MI-2 or doxorubicin analyzed by qRT-PCR. Wild-type embryos treated with DMSO were used as a control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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