Fig. 3

Effect of intravenous puromycin injection at 3 dpf on GFB structure (n = 3–4).(A) Image analysis of GFB for determination of podocyte effacement. Black double line = total length of GBM. White lines = foot process width. (B, C) Representative transmission electron microscopy micrographs at 24 hpi showing normal GFB structure with primary (*) and secondary foot processes with slit diaphragms (arrows). Foot process morphology is not significantly different from that of sham fish at 8 hpi (data not shown). (D, E) 25 mg mL⁻¹ puromycin results in patchy podocyte effacement (white brackets). (F) Administration of 25 mg mL⁻¹ puromycin significantly decreases the mean (±SEM) number of foot processes per unit length of GBM (p = 0.005) and (G) mean (±SEM) foot process width (p = 0.04). Analysis by one-way ANOVA with post hoc Dunnet's test. Scale bars are all 0.5 μm. GFB, glomerular filtration barrier; GBM, glomerular basement membrane. p-value summary: * < 0.05; ** ≤ 0.002.