Fig. S5

Histology of testis from wildtype (ar ^+/+), and homozygous (ar ^-/-) (the mutant 1) zebrafish at 1.5 mpf, 70dpf and 5 mpf. (A, B) Histology of testes from the wildtype (ar ^+/+) and homozygous (ar ^-/-) (ar ^{ihb1225/ihb1225}) zebrafish at 1.5 mpf. No obvious difference between ar ^-/- and wildtype sibling (ar ^+/+) testes. SGC, spermatogonial cysts (indicated with red arrows). (C, D) Histology of testes from the wildtype (ar ^+/+) and homozygous (ar ^-/-) (ar ^{ihb1225/ihb1225}) zebrafish at 70 dpf. Compared with wildtype sibling testes, spermatogenesis of ar^-/- testes was delayed as indicated by more spermatogonia (SG) and fewer spermatocytes (SC), but no spermatozoa (SZ). (E, F) Histology of testes from the wildtype (ar ^+/+) and homozygous (ar ^-/-) zebrafish at 6 mpf. ar ^-/- testes were degenerated and loose; but spermatozoa were filled in wildtype sibling (ar ^+/+) testes. Dpf, days post fertilization; Mpf, months post fertilization.